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KMID : 0380620130450010090
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Korean Journal of Food Science and Technology
2013 Volume.45 No. 1 p.90 ~ p.96
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Inhibitory Effect of Rumex Crispus L. Fraction on Adipocyte Differentiation in 3T3-L1 Cells
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Park Sung-Jin
Choi Jun-Hyeok
Jeong Yeon-Seop
Yu Me-Hee
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Abstract
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The anti-obesity effect of ethanol extract and their fractions from Rumex Crispus L. on the differentiation of
3T3-L1 pre-adipocytes to adipocytes was investigated by suppressing adipocyte differentiation and lipid accumulation with
Oil red O assay, western blot and real-time PCR analysis. Ethyl acetate fraction of Rumex crispus L. significantly inhibited
adipocyte differentiation when treated during the adipocyte differentiation process, as assessed by measuring fat
accumulation using Oil red O staining. In inducing differentiation of 3T3-L1 preadipocytes in the presence of an
adipogenic cocktail, isobutylmethylxanthine (IBMX), dexamethasone- and insulin- along with ethyl acetate fraction residue
processing treatment significantly decreased protein expression of obesity-related proteins, such as peroxisome-proliferatorsactivated-
receptor-γ (PPARγ) and CCAAT enhancer-binding-proteins α (C/EBPα). These results indicate that ethyl acetate
fraction of Rumex crispus L. is the most effective candidate for preventing obesity. However further studies will be needed
to identify the active compounds that confer the anti-obesity activity of ethyl acetate fraction from Rumex crispus L.
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KEYWORD
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Rumex Crispus L., adipocytes, PPARγ, C/EBPα, SREBP1c
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